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rmil 18  (R&D Systems)


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    Structured Review

    R&D Systems rmil 18
    Rmil 18, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rmil-18/Recombinant+Mouse+IL-12+Protein/pmc09043616__mmc2-233-163-167
    Average 95 stars, based on 267 article reviews
    rmil 18 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Article Snippet: Annamaria Biroccio1,2,18,20, Julien Cherfils-Vicini3,18, Adeline Augereau1,3,18, Sébastien Pinte1,19, Serge Bauwens1, Jing Ye1,3,4, Thomas Simonet1, Béatrice Horard1, Karine Jamet3, Ludovic Cervera3, Aaron Mendez-Bermudez3, Delphine Poncet1,19, Renée Grataroli1, Claire T’kint de Rodenbeeke1, Erica Salvati2, Angela Rizzo2, Pasquale Zizza2, Michelle Ricoul5, Céline Cognet6,7, Thomas Kuilman8, Helene Duret9, Florian Lépinasse10,11, Jacqueline Marvel12, Els Verhoeyen13, François-Loïc Cosset13, Daniel Peeper8, Mark J. Smyth9,14, Arturo Londoño-Vallejo15, Laure Sabatier5, Vincent Picco3, Gilles Pages3, Jean-Yves Scoazec10,11, Antonella Stoppacciaro16, Carlo Leonetti2, Eric Vivier6,7 and Eric Gilson1,3,17,20

    Article Title: The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4.
    Article Snippet: Used stimuli were recombinant murine (rm) IL-12 (BD PharMingen, San Diego, CA; R&D Systems, Minneapolis, MN), recombinant human TGF-b1, rmIL-4, rmIL11, rmIL-17, rmIL-18 (all from R&D Systems), IFN-g (kindly provided from Dr. G. Adolf at the Ernst Boehringer Institut, Vienna, Austria), and LPS (O111:B4; Sigma).

    Article Title: STAT5 Is a Key Regulator in NK Cells and Acts as a Molecular Switch from Tumor Surveillance to Tumor Promotion
    Article Snippet: Running title: NK cells as tumor promoters

    Article Title: NKT cells mediate organ-specific resistance against Leishmania major infection.
    Article Snippet: Whereas the acquired T cell-mediated protection against intracellular pathogens such as Leishmania major has been well studied in the past, the cells and mechanisms involved in their innate control are still poorly understood.. Here, we investigated the role of natural killer T (NKT) cells in a high dose L. major mouse infection model.. In vitro, L. major only weakly stimulated NKT cells and antagonized their response to the prototypic NKT cell ligand a-galactosylceramide, indicating that L. major partially escapes the activation of NKT cells.

    Article Title: METTL3-mediated m 6 A RNA methylation promotes the anti-tumour immunity of natural killer cells
    Article Snippet: To investigate the influence of cytokines on METTL3 expression in NK cells, we treated splenocytes with rmIL-17F (25 ng/mL, Peprotech), rmIL-27 (25 ng/mL, Peprotech), rmIL-10 (25 ng/mL, Peprotech), rmIL-15 (25 ng/mL, Peprotech), rmTGF-β (25 ng/mL, R&D) and rmIL-18 (25 ng/mL, R&D) for 3 days.

    Article Title: Control of Leishmania major in the absence of Tyk2 kinase.
    Article Snippet: Lymph node cells were restimulated with rmIL-12 (0.1–10 ng/ml; R&D Systems), rmIL-18 (0.1–10 ng/ml; R&D Systems), Leishmania antigen (freezethaw lysates of promastigotes; parasite:cell ratio =5:1) or concanavalin A (ConA; 2.5 ? g/ml, Sigma) in complete RPMI1640 medium with 5% FCS for 24 to 72 h. Culture supernatants were analyzed for IFN- + by ELISA (BD Biosciences and R&D Systems, sensitivity 50–100 pg/ml).

    Transformation Assay:

    Article Title: TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Article Snippet: Annamaria Biroccio1,2,18,20, Julien Cherfils-Vicini3,18, Adeline Augereau1,3,18, Sébastien Pinte1,19, Serge Bauwens1, Jing Ye1,3,4, Thomas Simonet1, Béatrice Horard1, Karine Jamet3, Ludovic Cervera3, Aaron Mendez-Bermudez3, Delphine Poncet1,19, Renée Grataroli1, Claire T’kint de Rodenbeeke1, Erica Salvati2, Angela Rizzo2, Pasquale Zizza2, Michelle Ricoul5, Céline Cognet6,7, Thomas Kuilman8, Helene Duret9, Florian Lépinasse10,11, Jacqueline Marvel12, Els Verhoeyen13, François-Loïc Cosset13, Daniel Peeper8, Mark J. Smyth9,14, Arturo Londoño-Vallejo15, Laure Sabatier5, Vincent Picco3, Gilles Pages3, Jean-Yves Scoazec10,11, Antonella Stoppacciaro16, Carlo Leonetti2, Eric Vivier6,7 and Eric Gilson1,3,17,20

    Article Title: The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4.
    Article Snippet: Used stimuli were recombinant murine (rm) IL-12 (BD PharMingen, San Diego, CA; R&D Systems, Minneapolis, MN), recombinant human TGF-b1, rmIL-4, rmIL11, rmIL-17, rmIL-18 (all from R&D Systems), IFN-g (kindly provided from Dr. G. Adolf at the Ernst Boehringer Institut, Vienna, Austria), and LPS (O111:B4; Sigma).

    Article Title: STAT5 Is a Key Regulator in NK Cells and Acts as a Molecular Switch from Tumor Surveillance to Tumor Promotion
    Article Snippet: Running title: NK cells as tumor promoters

    Article Title: NKT cells mediate organ-specific resistance against Leishmania major infection.
    Article Snippet: Whereas the acquired T cell-mediated protection against intracellular pathogens such as Leishmania major has been well studied in the past, the cells and mechanisms involved in their innate control are still poorly understood.. Here, we investigated the role of natural killer T (NKT) cells in a high dose L. major mouse infection model.. In vitro, L. major only weakly stimulated NKT cells and antagonized their response to the prototypic NKT cell ligand a-galactosylceramide, indicating that L. major partially escapes the activation of NKT cells.

    Article Title: METTL3-mediated m 6 A RNA methylation promotes the anti-tumour immunity of natural killer cells
    Article Snippet: To investigate the influence of cytokines on METTL3 expression in NK cells, we treated splenocytes with rmIL-17F (25 ng/mL, Peprotech), rmIL-27 (25 ng/mL, Peprotech), rmIL-10 (25 ng/mL, Peprotech), rmIL-15 (25 ng/mL, Peprotech), rmTGF-β (25 ng/mL, R&D) and rmIL-18 (25 ng/mL, R&D) for 3 days.

    Article Title: Control of Leishmania major in the absence of Tyk2 kinase.
    Article Snippet: Lymph node cells were restimulated with rmIL-12 (0.1–10 ng/ml; R&D Systems), rmIL-18 (0.1–10 ng/ml; R&D Systems), Leishmania antigen (freezethaw lysates of promastigotes; parasite:cell ratio =5:1) or concanavalin A (ConA; 2.5 ? g/ml, Sigma) in complete RPMI1640 medium with 5% FCS for 24 to 72 h. Culture supernatants were analyzed for IFN- + by ELISA (BD Biosciences and R&D Systems, sensitivity 50–100 pg/ml).

    Infection:

    Article Title: TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Article Snippet: Annamaria Biroccio1,2,18,20, Julien Cherfils-Vicini3,18, Adeline Augereau1,3,18, Sébastien Pinte1,19, Serge Bauwens1, Jing Ye1,3,4, Thomas Simonet1, Béatrice Horard1, Karine Jamet3, Ludovic Cervera3, Aaron Mendez-Bermudez3, Delphine Poncet1,19, Renée Grataroli1, Claire T’kint de Rodenbeeke1, Erica Salvati2, Angela Rizzo2, Pasquale Zizza2, Michelle Ricoul5, Céline Cognet6,7, Thomas Kuilman8, Helene Duret9, Florian Lépinasse10,11, Jacqueline Marvel12, Els Verhoeyen13, François-Loïc Cosset13, Daniel Peeper8, Mark J. Smyth9,14, Arturo Londoño-Vallejo15, Laure Sabatier5, Vincent Picco3, Gilles Pages3, Jean-Yves Scoazec10,11, Antonella Stoppacciaro16, Carlo Leonetti2, Eric Vivier6,7 and Eric Gilson1,3,17,20

    Article Title: The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4.
    Article Snippet: Used stimuli were recombinant murine (rm) IL-12 (BD PharMingen, San Diego, CA; R&D Systems, Minneapolis, MN), recombinant human TGF-b1, rmIL-4, rmIL11, rmIL-17, rmIL-18 (all from R&D Systems), IFN-g (kindly provided from Dr. G. Adolf at the Ernst Boehringer Institut, Vienna, Austria), and LPS (O111:B4; Sigma).

    Article Title: STAT5 Is a Key Regulator in NK Cells and Acts as a Molecular Switch from Tumor Surveillance to Tumor Promotion
    Article Snippet: Running title: NK cells as tumor promoters

    Article Title: NKT cells mediate organ-specific resistance against Leishmania major infection.
    Article Snippet: Whereas the acquired T cell-mediated protection against intracellular pathogens such as Leishmania major has been well studied in the past, the cells and mechanisms involved in their innate control are still poorly understood.. Here, we investigated the role of natural killer T (NKT) cells in a high dose L. major mouse infection model.. In vitro, L. major only weakly stimulated NKT cells and antagonized their response to the prototypic NKT cell ligand a-galactosylceramide, indicating that L. major partially escapes the activation of NKT cells.

    Article Title: METTL3-mediated m 6 A RNA methylation promotes the anti-tumour immunity of natural killer cells
    Article Snippet: To investigate the influence of cytokines on METTL3 expression in NK cells, we treated splenocytes with rmIL-17F (25 ng/mL, Peprotech), rmIL-27 (25 ng/mL, Peprotech), rmIL-10 (25 ng/mL, Peprotech), rmIL-15 (25 ng/mL, Peprotech), rmTGF-β (25 ng/mL, R&D) and rmIL-18 (25 ng/mL, R&D) for 3 days.

    Article Title: Control of Leishmania major in the absence of Tyk2 kinase.
    Article Snippet: Lymph node cells were restimulated with rmIL-12 (0.1–10 ng/ml; R&D Systems), rmIL-18 (0.1–10 ng/ml; R&D Systems), Leishmania antigen (freezethaw lysates of promastigotes; parasite:cell ratio =5:1) or concanavalin A (ConA; 2.5 ? g/ml, Sigma) in complete RPMI1640 medium with 5% FCS for 24 to 72 h. Culture supernatants were analyzed for IFN- + by ELISA (BD Biosciences and R&D Systems, sensitivity 50–100 pg/ml).

    Purification:

    Article Title: TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Article Snippet: Annamaria Biroccio1,2,18,20, Julien Cherfils-Vicini3,18, Adeline Augereau1,3,18, Sébastien Pinte1,19, Serge Bauwens1, Jing Ye1,3,4, Thomas Simonet1, Béatrice Horard1, Karine Jamet3, Ludovic Cervera3, Aaron Mendez-Bermudez3, Delphine Poncet1,19, Renée Grataroli1, Claire T’kint de Rodenbeeke1, Erica Salvati2, Angela Rizzo2, Pasquale Zizza2, Michelle Ricoul5, Céline Cognet6,7, Thomas Kuilman8, Helene Duret9, Florian Lépinasse10,11, Jacqueline Marvel12, Els Verhoeyen13, François-Loïc Cosset13, Daniel Peeper8, Mark J. Smyth9,14, Arturo Londoño-Vallejo15, Laure Sabatier5, Vincent Picco3, Gilles Pages3, Jean-Yves Scoazec10,11, Antonella Stoppacciaro16, Carlo Leonetti2, Eric Vivier6,7 and Eric Gilson1,3,17,20

    Article Title: The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4.
    Article Snippet: Used stimuli were recombinant murine (rm) IL-12 (BD PharMingen, San Diego, CA; R&D Systems, Minneapolis, MN), recombinant human TGF-b1, rmIL-4, rmIL11, rmIL-17, rmIL-18 (all from R&D Systems), IFN-g (kindly provided from Dr. G. Adolf at the Ernst Boehringer Institut, Vienna, Austria), and LPS (O111:B4; Sigma).

    Article Title: STAT5 Is a Key Regulator in NK Cells and Acts as a Molecular Switch from Tumor Surveillance to Tumor Promotion
    Article Snippet: Running title: NK cells as tumor promoters

    Article Title: NKT cells mediate organ-specific resistance against Leishmania major infection.
    Article Snippet: Whereas the acquired T cell-mediated protection against intracellular pathogens such as Leishmania major has been well studied in the past, the cells and mechanisms involved in their innate control are still poorly understood.. Here, we investigated the role of natural killer T (NKT) cells in a high dose L. major mouse infection model.. In vitro, L. major only weakly stimulated NKT cells and antagonized their response to the prototypic NKT cell ligand a-galactosylceramide, indicating that L. major partially escapes the activation of NKT cells.

    Article Title: METTL3-mediated m 6 A RNA methylation promotes the anti-tumour immunity of natural killer cells
    Article Snippet: To investigate the influence of cytokines on METTL3 expression in NK cells, we treated splenocytes with rmIL-17F (25 ng/mL, Peprotech), rmIL-27 (25 ng/mL, Peprotech), rmIL-10 (25 ng/mL, Peprotech), rmIL-15 (25 ng/mL, Peprotech), rmTGF-β (25 ng/mL, R&D) and rmIL-18 (25 ng/mL, R&D) for 3 days.

    Article Title: Control of Leishmania major in the absence of Tyk2 kinase.
    Article Snippet: Lymph node cells were restimulated with rmIL-12 (0.1–10 ng/ml; R&D Systems), rmIL-18 (0.1–10 ng/ml; R&D Systems), Leishmania antigen (freezethaw lysates of promastigotes; parasite:cell ratio =5:1) or concanavalin A (ConA; 2.5 ? g/ml, Sigma) in complete RPMI1640 medium with 5% FCS for 24 to 72 h. Culture supernatants were analyzed for IFN- + by ELISA (BD Biosciences and R&D Systems, sensitivity 50–100 pg/ml).

    Spectrophotometry:

    Article Title: TRF2 inhibits a cell-extrinsic pathway through which natural killer cells eliminate cancer cells.
    Article Snippet: Annamaria Biroccio1,2,18,20, Julien Cherfils-Vicini3,18, Adeline Augereau1,3,18, Sébastien Pinte1,19, Serge Bauwens1, Jing Ye1,3,4, Thomas Simonet1, Béatrice Horard1, Karine Jamet3, Ludovic Cervera3, Aaron Mendez-Bermudez3, Delphine Poncet1,19, Renée Grataroli1, Claire T’kint de Rodenbeeke1, Erica Salvati2, Angela Rizzo2, Pasquale Zizza2, Michelle Ricoul5, Céline Cognet6,7, Thomas Kuilman8, Helene Duret9, Florian Lépinasse10,11, Jacqueline Marvel12, Els Verhoeyen13, François-Loïc Cosset13, Daniel Peeper8, Mark J. Smyth9,14, Arturo Londoño-Vallejo15, Laure Sabatier5, Vincent Picco3, Gilles Pages3, Jean-Yves Scoazec10,11, Antonella Stoppacciaro16, Carlo Leonetti2, Eric Vivier6,7 and Eric Gilson1,3,17,20

    Article Title: The production of IFN-gamma by IL-12/IL-18-activated macrophages requires STAT4 signaling and is inhibited by IL-4.
    Article Snippet: Used stimuli were recombinant murine (rm) IL-12 (BD PharMingen, San Diego, CA; R&D Systems, Minneapolis, MN), recombinant human TGF-b1, rmIL-4, rmIL11, rmIL-17, rmIL-18 (all from R&D Systems), IFN-g (kindly provided from Dr. G. Adolf at the Ernst Boehringer Institut, Vienna, Austria), and LPS (O111:B4; Sigma).

    Article Title: STAT5 Is a Key Regulator in NK Cells and Acts as a Molecular Switch from Tumor Surveillance to Tumor Promotion
    Article Snippet: Running title: NK cells as tumor promoters

    Article Title: NKT cells mediate organ-specific resistance against Leishmania major infection.
    Article Snippet: Whereas the acquired T cell-mediated protection against intracellular pathogens such as Leishmania major has been well studied in the past, the cells and mechanisms involved in their innate control are still poorly understood.. Here, we investigated the role of natural killer T (NKT) cells in a high dose L. major mouse infection model.. In vitro, L. major only weakly stimulated NKT cells and antagonized their response to the prototypic NKT cell ligand a-galactosylceramide, indicating that L. major partially escapes the activation of NKT cells.

    Article Title: METTL3-mediated m 6 A RNA methylation promotes the anti-tumour immunity of natural killer cells
    Article Snippet: To investigate the influence of cytokines on METTL3 expression in NK cells, we treated splenocytes with rmIL-17F (25 ng/mL, Peprotech), rmIL-27 (25 ng/mL, Peprotech), rmIL-10 (25 ng/mL, Peprotech), rmIL-15 (25 ng/mL, Peprotech), rmTGF-β (25 ng/mL, R&D) and rmIL-18 (25 ng/mL, R&D) for 3 days.

    Article Title: Control of Leishmania major in the absence of Tyk2 kinase.
    Article Snippet: Lymph node cells were restimulated with rmIL-12 (0.1–10 ng/ml; R&D Systems), rmIL-18 (0.1–10 ng/ml; R&D Systems), Leishmania antigen (freezethaw lysates of promastigotes; parasite:cell ratio =5:1) or concanavalin A (ConA; 2.5 ? g/ml, Sigma) in complete RPMI1640 medium with 5% FCS for 24 to 72 h. Culture supernatants were analyzed for IFN- + by ELISA (BD Biosciences and R&D Systems, sensitivity 50–100 pg/ml).



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